Debottlenecking of an In Vitro Enzyme Cascade Using a Combined Model‐ and Experiment‐Based Approach

Abstract Designing a feasible in vitro enzyme cascade is challenging task that often preceded by optimization steps. Computational methods can increase the efficiency of process providing deeper insight into system. In this study, bottlenecks farnesyl pyrophosphate‐producing were determined combination silico modeling and wet‐lab experiments. ATP precursor‐isomerizing isopentenyl diphosphate δ ‐isomerase identified as limitations cascade.